developmental consequences of mouse cryotop-vitrified oocyte and embryo using low concentrated cryoprotectants
نویسندگان
چکیده
background: the risk of multiple pregnancies often present in programs of in vitro fertilization (ivf) is an important force for embryo cryopreservation. on the other hand ethical restriction and assurance of potential fertility following chemo/radio therapy has led scientists to focus on female gamete preservation. objective: optimizing vitrification protocol by using less concentrated cryoprotectants (cpas) in order to decrease cpas toxicity. materials and methods: mouse metaphase-ii (m-ii) oocytes and four cell-stage embryos were collected. oocytes survival fertilization and developmental rates (srs frs drs) were recorded after cryotop-vitrification/warming. as well as comparing fresh oocytes and embryos the data obtained from experimental groups (exp.) applying 1.25 1.0 0.75 molar (m) cpas were analyzed in comparison to those of adopting 1.5 m cpas [largely-used concentration of ethylen glycol (eg) and dimethyl-sulphoxide (dmso)]. results: the data of oocytes exposed to 1.25 m concentrated cpas were in consistency with those exposed to 1.5 m and control group in terms of sr fr and dr. as less concentration was applied the more decreased srs frs and drs were obtained from other experimental groups. the results of embryos which were exposed to 1.25 m and 1.0 m were close to those vitrified with 1.5 m and fresh embryos. the results of 0.75 m concentrated cpas solutions were significantly lower than those of control 1.5 m and 1.0 m treated groups. conclusion: cpas limited reduction to 1.25 m and 1.0 m instead of using 1.5 m for oocyte and embryo cryotop-vitrification procedure may be a slight adjustment.
منابع مشابه
Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability
متن کامل
Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability
OBJECTIVE(S) The examination of the possibility of applying lower CPA- concentrations and obtaining the similar results to those using higher concentrations; as it is shown, the toxicity of the CPAs used in vitrification approach will diminish. MATERIALS AND METHODS Following vitrification/warming, oocytes were subjected to PZD/ICSI. SRs, FRs, and DRs were recorded. SRs and DRs of the embryos...
متن کاملmouse oocytes and embryos cryotop-vitrification using low concentrated solutions: effects on meiotic spindle, genetic material array and developmental ability
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عنوان ژورنال:
international journal of reproductive biomedicineجلد ۷، شماره ۴، صفحات ۱۸۱-۰
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